Internal Standard Quantitation

[lnicolot]

Hallo,
I am working using SIDA (Standard Isotope Dilution Analysis), is it possible to perform the "internal standard quantitation" using the ratio between qualifiuer and quantifier instead of "volume" and "1st quantifier" responses?

[qtao]

No, not right now although we are planning to support isotope dilution in the future. Currently a possible workaround is either to obtain the calibration function externally and then compute the quantities with Analysis CLIC of a blob, or use an area object overlapping with a blob. Both are a little bit tricky.

[sarakhan]

Is it possible to visualize more than 4 quantifiers or qualifiers in the blob table?

[qtao]

You can add CLIC columns to show more quantifiers and qualifiers. For example, CLIC "Quantifier[5]" will show the 5th quantifier.

Please see here for details on how to add a CLIC column to Blob Table:
http://www.gcimage.com/gcxgc/usersguide ... lobalClics

[AZhou]

Hello,

I have the similar problem with SIDA setup. You mentioned that it is doable by "using an area object overlapping with a blob". Could you give some detail instructions about the procedures? Thanks so much.

azhou

[qtao]

For R2.5, the area-object work-around is :

1. Create an area object overlapping with the blob
(http://www.gcimage.com/gcxgc/usersguide ... sFromBlobs)

2. Set the blob as ISTD

3. Specify different names for the blob and area object, for example, Blob is "Benzene-ISTD", Area is "Benzene".

4. Specify quantifier ions for the area object and the blob
(http://www.gcimage.com/gcxgc/usersguide ... #qionTable)

5. Quantify with the area object as usual
(http://www.gcimage.com/gcxgc/usersguide ... l#quantIon)

Note that the above workaround may or may not work for you, which depends on the calibration/quantitation equations you want to use.