GC Image LCxLC Edition Users' Guide

New Features and Improvements in Release 2.3

GC Image LCxLC Edition Release 2.3 (August 2012) has many new features and improvements. See the GC Image LCxLC Edition Users' Guide for full documentation. GC Image LCxLC Edition Release 2.3 requires Microsoft Windows XP Service Pack 3 or later operating system.

Spectral Background Subtraction

GC Image LCxLC Edition R2.3 introduces new tools for subtracting a background spectrum from a chromatogram. This release supports:

• Subtraction of a spectrum from the entire chromatogram or within selected graphical regions.
• Optional normalization of the subtracted spectrum based on its base peak, its ten largest peaks, all its peaks, or its peaks in user-defined spectral range(s).
• Specifying a spectral tolerance to operate on peaks with sub-integer mass precision for subtraction (available in HRMS Edition software only).

Improved Blob Table and Blob Set Table

GC Image LCxLC Edition R2.3 updates the Blob Table and Blob Set Table with new features and configuration options.

• The Blob Set Table now supports aggregated values for all computed attributes of blobs except for CLIC columns.
• Users can configure how to compute aggregated values in the Blob Set Table with common aggregators including min, max, sum, and average.
• Users can configure the Blob Set Table to show multiple aggregated values for a single computed attribute.
• Users can configure column order directly on the Blob Table and Blob Set Table by drag-and-drop.

Improved CLIC Expression tool with JavaScript Support

GC Image LCxLC Edition R2.3 significantly expands the functions and flexibility of CLIC with JavaScript support. This release also updates the CLIC Expression tool with a new interface and expanded functionality.

• CLIC now supports custom functions written as JavaScript plug-ins.
• CLIC supports a new function, FeatureAtBlob(), that allows access to computed attributes of other blobs.
• The CLIC Expression tool supports exporting and importing expressions.
• The CLIC Expression tool displays the most recently used functions for convenient access.
• The CLIC Expression tool displays the description of a function upon selection.

Image Investigator™ with Multi-Class Analysis and Visual Feature Selection

GC Image LCxLC Edition R2.3 adds new support for multi-class analysis and feature selection to Image Investigator. This release supports:

• Principal component analysis (PCA) with any attribute for unsupervised class analysis.
• Reporting pairwise differences, pairwise Fisher ratios, and one-vs-all Fisher ratios of all attributes for supervised multi-class analysis.
• Interactive line, scatter, bubble, and principal component plots for examining and selecting features.
• Filtering features based on computed statistics in tables and plots.
• Saving plots and tables to XML, CSV, and XLS files for reporting or external analysis.

In addition, Image Investigator supports:

• Options for relaxing the constraints for reliable peaks in automated feature template generation.
• Remembering paths and user preferences across sessions.

Automated Processing with Methods

GC Image LCxLC Edition R2.3 adds more fully featured support for automated data processing methods. In addition to support for exporting a method from a processed image, this release supports:

• Building a method with processing steps in a custom order.
• Allowing a method to have parameters specified at runtime for more flexibility.
• Running a method on any processed or unprocessed image.
• Exporting, importing, and managing multiple methods.

Batch Processing with Workflows

GC Image LCxLC Edition R2.3 introduces support for workflows in GC Project. In addition to the New Project Wizard, this release supports:

• Three additional built-in workflows for common tasks including processing with a sequence table, existing runs, and existing images.
• Recording a custom workflow based on built-in workflows.
• Running a workflow on an existing project at any time.
• Exporting, importing, managing, and editing multiple workflows.

New and Improved Operations and I/O Support

GC Image LCxLC Edition R2.3 has new and improved I/O facilities and other improvements, including:

• Login with the current MS Windows user account.
• The 1D View tool now also contains a static view in addition to the interactive view.
• Importing and merging chromatograms in multiple data files, e.g., one chromatographic file per secondary separation.
• Exporting data into other user-defined text formats implemented with JavaScript plug-ins.
• Working with profile spectra in MassHunter data files (available in HRMS Edition software only).
• Support for ALMSCO's BenchTOF files.

Contents Next: Introduction

GC Image™ LCxLC Edition Users' Guide © 2001–2012 by GC Image, LLC, and the University of Nebraska.