Internal Standard Quantitation

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lnicolot
Posts: 14
Joined: January 23rd, 2014, 3:39 am

Internal Standard Quantitation

Post by lnicolot » July 4th, 2014, 2:55 am

Hallo,
I am working using SIDA (Standard Isotope Dilution Analysis), is it possible to perform the "internal standard quantitation" using the ratio between qualifiuer and quantifier instead of "volume" and "1st quantifier" responses?

qtao
Posts: 148
Joined: October 7th, 2011, 10:16 am

Re: Internal Standard Quantitation

Post by qtao » July 4th, 2014, 7:01 pm

No, not right now although we are planning to support isotope dilution in the future. Currently a possible workaround is either to obtain the calibration function externally and then compute the quantities with Analysis CLIC of a blob, or use an area object overlapping with a blob. Both are a little bit tricky.

sarakhan
Posts: 1
Joined: October 31st, 2014, 1:58 am

Re: Internal Standard Quantitation

Post by sarakhan » October 31st, 2014, 2:00 am

Is it possible to visualize more than 4 quantifiers or qualifiers in the blob table?
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qtao
Posts: 148
Joined: October 7th, 2011, 10:16 am

Re: Internal Standard Quantitation

Post by qtao » November 5th, 2014, 10:43 am

You can add CLIC columns to show more quantifiers and qualifiers. For example, CLIC "Quantifier[5]" will show the 5th quantifier.

Please see here for details on how to add a CLIC column to Blob Table:
http://www.gcimage.com/gcxgc/usersguide ... lobalClics

AZhou
Posts: 1
Joined: February 24th, 2015, 8:32 am

Re: Internal Standard Quantitation

Post by AZhou » June 10th, 2015, 3:08 pm

Hello,

I have the similar problem with SIDA setup. You mentioned that it is doable by "using an area object overlapping with a blob". Could you give some detail instructions about the procedures? Thanks so much.

azhou

qtao
Posts: 148
Joined: October 7th, 2011, 10:16 am

Re: Internal Standard Quantitation

Post by qtao » June 11th, 2015, 1:55 pm

For R2.5, the area-object work-around is :

1. Create an area object overlapping with the blob
(http://www.gcimage.com/gcxgc/usersguide ... sFromBlobs)

2. Set the blob as ISTD

3. Specify different names for the blob and area object, for example, Blob is "Benzene-ISTD", Area is "Benzene".

4. Specify quantifier ions for the area object and the blob
(http://www.gcimage.com/gcxgc/usersguide ... #qionTable)

5. Quantify with the area object as usual
(http://www.gcimage.com/gcxgc/usersguide ... l#quantIon)

Note that the above workaround may or may not work for you, which depends on the calibration/quantitation equations you want to use.

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