GC IMAGE - GC Image LCxLC Edition - User Guide 2025R1

New Features and Improvements in Version 2025

GC Image LCxLC Edition 2025 has many new features and improvements. See the GC Image LCxLC Edition User Guide for full documentation.

What's New in Version 2025 Release 1 (June 2025)

Improved Ion Peak Detection Algorithm with Better Reliability

Version 2025R1 includes a significant update to the ion peak detection algorithm with improving reliability and precision. This updated algorithm enhances ion peak merging and noisy peak filtering, delivering more accurate peak detection and deconvolved spectra. It replaces the previous version and is now the default.

Faster Performance for CLIC Columns in Blob Table

In Version 2025R1, calculations using CLIC functions, FEATUREATBLOB() and IDOF(), especially when referencing a specific identified blob like an ISTD, now run faster than ever. This enhanced speed allows for more complex and flexible normalization and calculations, for example, Volume/FEATUREATBLOB(IDOF("Biphenyl-d10", "Blob"), "Volume"), to be executed smoothly in Blob Table.

Enhanced Alignment Marker Selection for Compare Images Tool

Version 2025R1 introduces a smarter alignment marker selection process in the Compare Images Tool, enhancing accuracy and reliability. The updated algorithm refines initial matching by pairing identically named blobs across images while prioritizing consistency with the predicted transform. This ensures the most stable and globally reliable transformation.

Additionally, a new Only Use Included option allows users to exclusively utilize included blobs when initializing alignment markers, providing greater control over the selection process.

New Difference View for Side-by-Side Chromatogram Comparison

Version 2025R1 introduces Difference View, a powerful tool designed to quickly identify and analyze differences between two chromatograms. This directional comparison computes a pixel-by-pixel difference, highlighting what is present in the first operand image but absent or reduced in the second.

This new Difference View leverages precise alignment using alignment markers and transforms, and offers advanced options including:

Normalization: Adjusts how the second image is normalized to the first.
Fuzzy Radius: Optimizes pixel matching and counters local variations for improved accuracy.
Relative Difference and SNR Filtering: Select None, % Recovery, or Fold Change, to refine results while accounting for baseline fluctuations.

In addition, a dedicated Review table displays differentiating compounds, either matched or unmatched, that have been reviewed and added for further analysis.

Enhanced Tools and Streamlined Interface for Multi-Sample Analysis

Version 2025R1 introduces new analysis tools in the Investigator interface including:

Integrated Compare Images Support within Analysis: Now seamlessly integrate the Compare Images tool directly within the Analysis Actions. This integration enables a more comprehensive comparison of two samples by combining analytical insights with direct visual inspection of chromatograms and peaks, ensuring thorough evaluations.
New Pre-Configured Analysis Tools:
- Compare Classes: Generate a bubble plot of the mean pairwise class differences for a specified attribute.
- Run PCA: Perform principal component analysis using the selected attribute for unsupervised clustering.
- Run F-Test: Create bubble plots displaying F-values to evaluate compound significance for distinguishing classes.

In addition, Version 2025R1 introduces a refreshed interface for Investigator to optimize usability and visualization:

Better Chromatogram View displays a sharper and more visually appealing chromatogram snapshot at any viewing size.
Redesigned Analysis Navigation uses the new side-bar system, replacing the old tab-based interface for a more organized and intuitive experience.

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